DNA Fragmentation Index (DFI)
At Eva IVF & Women’s Centre, we understand that successful conception depends on more than just sperm count or motility. One critical yet often overlooked factor is the integrity of the sperm’s DNA. Our advanced DNA Fragmentation Index (DFI) test offers deep insights into sperm DNA quality, helping couples struggling with unexplained infertility or repeated IVF/IUI failures find answers—and solutions.
Whether you’re planning natural conception or assisted reproductive treatment, the DFI test can be a game-changer in identifying hidden barriers to pregnancy success.
What is DNA Fragmentation Index (DFI)?
The DNA Fragmentation Index (DFI) indicates the proportion of sperm with broken or impaired DNA strands in a semen sample, helping assess sperm quality and potential impact on fertility outcomes. Even if a man’s sperm count, motility, and morphology appear normal, elevated DNA fragmentation can affect the sperm’s ability to fertilize an egg or support healthy embryo development.
🔬 A high DFI means that a significant number of sperm have breaks or abnormalities in their genetic material, increasing the risk of:
• Poor fertilization outcomes
• Early embryo arrest
• Miscarriages
• IVF or ICSI failure
• Poor embryo quality
💡 DFI testing goes beyond standard semen analysis to uncover hidden male factor infertility.
Who Should Consider a DFI Test?
The DFI test is recommended for men who:
✔ Normal semen parameters may still lead to unexplained infertility due to hidden functional or genetic sperm issues.
✔ Have experienced recurrent IVF/ICSI failure or pregnancy loss
✔ Have lifestyle risk factors (e.g. smoking, stress, obesity, heat exposure)
✔ Are exposed to toxins, chemicals, or radiation at work
✔ Have varicocele, infections, or chronic illness
✔ Want a complete evaluation before undergoing fertility treatments
💡 Even men with healthy lifestyles can have DNA damage—only testing can reveal the truth.
How is the DFI Test Done at Eva IVF?
1. Sample Collection
• Sample can be provided at the clinic or using a secure take-home kit (if applicable)
2. Advanced Laboratory Analysis
• SCSA (Sperm Chromatin Structure Assay)
• COMET assay (Single-cell gel electrophoresis)
Our andrology lab processes the sample under strict timelines to preserve accuracy.
3. Result Interpretation
• Based on the score, your fertility expert will recommend next steps
Understanding Your DFI Score
DFI Range Interpretation Fertility Implications
<15% Normal DNA integrity Excellent fertilization potential
15–25% Moderate DNA fragmentation May impact embryo quality and pregnancy rates
>25% High DNA fragmentation Increased risk of miscarriage, IVF failure
💡 Higher DFI may require targeted treatment or specialized sperm selection methods for ART.
Can a High DFI Be Treated or Improved?
Yes, DNA fragmentation is often reversible with lifestyle changes or antioxidants. Based on your results, we may recommend:
• Antioxidant supplements (Vitamin C, E, CoQ10, Zinc, etc.)
• Lifestyle changes (diet, exercise, quitting smoking, reducing heat/stress exposure)
• Varicocele repair (if indicated)
• Shorter abstinence intervals before collection
• Advanced sperm selection techniques like PICSI, MACS, or Micro-TESE for ICSI
💬 We take a comprehensive approach—addressing both cause and solution.
Why Choose Eva IVF & Women’s Centre for DFI Testing?
🌟 Fertility-Focused Andrology Lab – Designed for accurate male fertility diagnostics
🌟 Advanced Testing Platforms (TUNEL, SCSA, COMET)
🌟 Quick Turnaround with Expert Consultation
🌟 Detailed Counseling Based on Results
🌟 Holistic Treatment Plans for DNA Recovery
🌟 Seamless Integration with IVF/ICSI Treatment
We offer more than just numbers—we offer direction, clarity, and a fertility roadmap that’s built around your unique situation.